生物知识 Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochon The MIA40 pathway is a novel import pathway in mitochondria specific for cysteine-... 04月17日 发表评论 阅读全文
生物知识 Nanodroplet Chemical Microarrays and Label-Free Assays The microarraying of chemicals or biomolecules on a glass surface allows for dense... 04月17日 发表评论 阅读全文
生物知识 Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Ph The minor inositol-containing membrane phospholipids, the phosphoinositides, play ... 04月17日 发表评论 阅读全文
生物知识 Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells The mitochondrial membrane potential is the dominant component of the proton-motiv... 04月17日 发表评论 阅读全文
生物知识 Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitoch The mitochondrial oxidative phosphorylation involves five multimeric complexes imb... 04月17日 发表评论 阅读全文
生物知识 Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells The mitogen-activated protein kinase (MAPK) signaling pathway is initiated by dive... 04月17日 发表评论 阅读全文
生物知识 Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum The model organismD. discoideumis well suited to investigate basic questions of mo... 04月17日 发表评论 阅读全文
生物知识 Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells The modulatory potency of lectins on cellular activities deserves attention from a... 04月17日 发表评论 阅读全文
生物知识 Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine The molecular diagnosis of hereditary and somatic disorders is a rapidly expanding... 04月17日 发表评论 阅读全文
生物知识 病毒红细胞凝集试验 原理有些病毒(如流感病毒等)表面有血凝素(为糖蛋白)在一定条件下,能与鸡,豚鼠的红细胞表面的糖蛋白受体结合而发生凝集现象。利用病毒血凝试验可以检测某些病毒的存在,滴... 04月17日 发表评论 阅读全文