Phenol (removes protein)1.add equal volume of Phenol (= tris-saturated Phenol-Chlo...

DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus be...

Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 ul of 10 ug Ge...

OverviewProcedure1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fresh...

1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapilla...

Protocol for extracting DNA from ES Cells, starting from the 96-well plate but pro...

Background on the art of getting beautiful Southerns:There are several things to c...

In the hood:96 well dish with bacteriatitertechmicrotubesglass pipetteRemove colon...

Northern assays require RNA (total- or poly(A)- selected) to be resolved on a agar...