一、简介利用一个假定的突变通过互补作用克隆酵母菌基因的一般方法。该突变为cdc101-1，它对酵母菌的生长来说既是隐性的又是温度敏感的。它使酵母可以很正常地在30°...
生物知识
生物知识
生物知识
虫媒病毒的直接检测法
简介虫媒病毒（ arbovirus）是指一些通过吸血节肢动物叮咬敏感脊椎动物而引起的自然疫源性疾病及人兽共患病的一群病毒，包括不同基因组的许多病毒家族，如黄病毒科（...
生物知识
硝酸银染色法
一、简介细菌的鞭毛很纤细，其直径通常为 0.01 ~0.02 μm， 所以，除了很少数能形成鞭毛束（由多根鞭毛构成）的细圈可以用相差显微镜直接观察到鞭毛束的存在外，...
生物知识
透射电镜超薄切片和染色实验
一、简介超薄切片技术 (Ultramicrotomy)是为透射电镜观察提供薄标本的专门技术，是生物学中研究细胞、组织超微结构最常用的技术，也是生物学中其它电子显微技...
生物知识
Antibody Affinity Maturation by Random Mutagenesis
The immune response to an antigen can be considered to occur in two stages. In the...
生物知识
In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polyp
The import of precursor proteins into mitochondria represents a cell biological pr...
生物知识
Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of
The importance of understanding the mechanisms of modulation of cellular signaling...
生物知识
Machine Learning: An Indispensable Tool in Bioinformatics
The increase in the number and complexity of biological databases has raised the n...
生物知识
Structural Genomics of Minimal Organisms: Pipeline and Results
The initial objective of the Berkeley Structural Genomics Center was to obtain a n...
生物知识
Nitric Oxide Imaging in Neurons Using Confocal Microscopy
The instability and low concentration of nitric oxide (NO) in specimens make it di...
