The bisulfite reaction reaction was first described in early 1970s and was used by...
生物知识
生物知识
生物知识
Nested RT-PCR for Hepatitis C from Paraffin Sections
RNA Extraction from Histologic SectionsUnstained 4 µm thick sections of formalin f...
生物知识
高GC比模板的PCR扩增
PCR条件的优化是一个麻烦耗时的过程，涉及到温度、时间、镁离子、引物、dNTP、Taq酶、模板等多个因素的调整。一般来说利用热启动，比如Platinum Taq D...
生物知识
Inverse PCR For use with Snyder mTn-lacZ/LEU2 based mutagenesis
Inverse PCR I. Genomic DNA Prep• from 5 ml culture, resuspend in 50 µl TEII. Diges...
生物知识
聚合酶链反应一单链构象多态性分析 (PCR-SSCP) 实验方法
聚合酶链反应一单链构象多态性分析 (PCR-SSCP) 聚合酶链反应-单链构象多态性分析（Single Strand Conformation Polymorp...
生物知识
Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR
1) Immunocapture stageCoating buffer: 15 mM Na2 CO3 ; 35 mM NaHCO3 , and 3 mM NaN3...
生物知识
RAPD技术应用中的一些问题及对策
焦锋，楼程富 （浙江大学蚕学系，杭州 310029） 摘要：综述了RAPD技术的一些理论性问题，包括RAPD与其它分子标记技术相比的优点，影响结果重复性的因素，显性...
生物知识
PCR and multiplex PCR guide
PCR and multiplex PCR guideTable below lists the parameters influencing the PCR re...
生物知识
Primer Design Rules for Bisulfite Conversion Based PCR
Primer Design Rules for Bisulfite Conversion Based PCRAuthor:Long-Cheng LiSource:P...
