I tried to clean a restriction product >10kb before ligation by a gel extractio...
生物知识
生物知识
生物知识
Large scale nuclear extract preparation
Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Karin...
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DNA and RNA EXTRACTIONS
A protocol / method / schedule /procedure for extraction / isolation of both DNA a...
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Phlebotomus Sandflies的DNA的提取
1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM...
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OLIGO PURIFICATION on acrylamide gels
GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1. Qu...
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Oligonucleotide Purification
Purpose: Purification of oligonucleotides (which have already been purified by rev...
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Purification of DNA from lambda phage
This is a modification of the procedure in Short Protocols (1-41,1-45)1.Prepare a ...
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Aninexpen sivealter native to glass milk for DNA purificatio
Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2. A...
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Rapid elution of DNA from agaro
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, Nov...
生物知识
Rapid elution of DNA from agarose gels
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, Nov...
