OverviewThis is a protocol for preparing whole cell lysate from tissue, for wester...
生物知识
生物知识
生物知识
Isolation of Non-muscle Actin
General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buffer...
生物知识
HISTONE KINASE ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot...
生物知识
WESTERN PROTOCOL
WESTERN PROTOCOLA. Preparation of cell lysatesCollect cells (confluent T-25) by tr...
生物知识
Affinity chromatography (with HIS-tagged proteins)
Affinity chromatography (with HIS-tagged proteins)This protocol is described in le...
生物知识
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots
Preparation of Nuclear Extracts for Gel Shifts and Westerns BlotsThe following pro...
生物知识
Preparation of Brain Membrane Fractions for Western Blot Analysis
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedure...
生物知识
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature ConditionsAccording to AMERSHAM-BIOS...
生物知识
Western 不同转膜方法的取舍
转膜: 小分子量的蛋白半干转效果比较好， 大分子量（100KD）以上建议湿转。 具体转膜条件需要多摸一下。30-80KD半干转恒流1mA/cm2，1-1.5h 都可...
生物知识
Western bloting 综述
摘要：本文主要是针对Western bloting的原理及操作进行了简单的阐述，Western印迹法是利用抗原抗体的免疫反应，先将蛋白通过SDS-PAGE电泳分离开...
