Western Blot MaterialsSemi-dry Transfer ApparatusBiorad, Cat# 170-3940, or equival...
生物知识
生物知识
生物知识
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
IntroductionThis is an adaptation of the published procedure for RNAse treatment o...
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Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditionsIntro...
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WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains s...
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ELISA FOR DETECTING SECRETED ICAM-1
PurposeMaterialsNUNC-Immuno Plate IIF.Horseradish Peroxidase-Streptavidin (Zymed; ...
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Home-made Taq Polymerase Purification
Home-made Taq Polymerase PurificationI also have the bacteria containing the clone...
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Protein Expression and Purification Protocol
Step 1:Transformappropriate DNA plasmid into BL21(DE3)E. colicells. These cells mu...
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GST Fusion Protein Prep
Italics indicate optional steps especially useful for the analysis of untested pro...
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Western Blotting with Monoclonal Antibodies
Sample PreparationFor Protein Concentration Determination of Cell CultureDecant me...
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Protein extraction from whole tissues for IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extractio...
