生物知识 A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts We describe a fluorescence assay for nitric oxide synthase activity based on a new... 04月16日 发表评论 阅读全文
生物知识 Protein Processing Characterized by a Gel-Free Proteomics Approach We describe a method for the specific isolation of representative N-terminal pepti... 04月16日 发表评论 阅读全文
生物知识 Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope We describe a new method for quantitative tissue proteomics using culture-derived ... 04月16日 发表评论 阅读全文
生物知识 Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extr... 04月16日 发表评论 阅读全文
生物知识 Systems for the Cell-Free Synthesis of Proteins We describe a system for the cell-free expression of proteins based on extracts fr... 04月16日 发表评论 阅读全文
生物知识 Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int We describe an alternative to the molecular biology technique of polyacrylamide ge... 04月16日 发表评论 阅读全文
生物知识 An antibody-lectin sandwich assay for quantifying protein glycoforms We describe an antibody-lectin sandwich assay for quantitation of glycoforms of pr... 04月16日 发表评论 阅读全文
生物知识 An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri We describe an improved, efficient and reliable method for the vapour-phase silani... 04月16日 发表评论 阅读全文
生物知识 Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction We describe here a method for analyzing a rat liver nuclear-insoluble protein frac... 04月16日 发表评论 阅读全文
生物知识 Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors We describe in detail the different steps involved in the construction of a carbon... 04月16日 发表评论 阅读全文