The regulation of exocytosis by Rho GTPases is conserved in eukaryotic kingdoms. G...
生物知识
生物知识
生物知识
Phosphoinositide (PI) 3-Kinase Assays
The regulation of phosphoinositide (PI) 3-kinase activities has been linked to man...
生物知识
The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotrop
The retinol carrier retinol-binding protein (RBP) forms in blood a complex with th...
生物知识
One-Tube RT-PCR with Sequence-Specific RT Primers
The reverse transcription-polymerase chain reaction (RT-PCR; 1 ,2 ) applies the po...
生物知识
Posttranslational Lipid Modification of Rho Family Small GTPases
The Rho family comprises a major branch of the Ras superfamily of small GTPases. A...
生物知识
RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer
The RNA electrophoretic mobility shift assay is a simple and rapid method for visu...
生物知识
S1 Mapping Using Single-Stranded DNA Probes
The S1 nuclease is an endonuclease isolated fromAspergillus oryzaethat digests sin...
生物知识
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
The Sanger dideoxy-nucleotide sequencing method has been simplified by a number of...
生物知识
Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for ...
生物知识
Messenger RNA Fractionation on Neutral Sucrose Gradients
The separation of RNA on the basis of size by sucrose gradient fractionation is a ...
