Purification of Gcn4James Fishburn,Hahn Lab March 2006Gcn4 constructs are cloned i...
生物知识
生物知识
生物知识
Yeast mini whole cell extract using glass beads for western analysis
Before trying this method,try rapid protein prep which involves boiling yeast in S...
生物知识
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1 liter cell prep1)gro...
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rat liver cytosol preperation
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle ...
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Preparation of Tonsil Lysate
PurpseThe prtcl describes hw t prepare human tnsil lysate fr use in purificatin f ...
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Bacterial Protein Extraction (mini-scale)
Bacterial Protein Extraction (mini-scale)Using B-PerB-Per (Pierce,Cat number: 7824...
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Purification of Myosin Light Chain Kinase
Solutions1.1 M Acetic Acid2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stock ...
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Isolation of Protein from Tissue
Place the tissues on labeled aluminum foil and immediately place in dry ice.It is ...
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Purification of Calmodulin from Bovine Brain
References:Watterson et al.(1980)J Biol Chem 255:962-75.Charbonneau et al.(1979)Bi...
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Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura M...
