生物知识 PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE) The methods discussed in this chapter were the first pulsed-field gel electrophore... 04月17日 发表评论 阅读全文
生物知识 Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers The methods for the synthesis and purification of negatively charged peptide nucle... 04月17日 发表评论 阅读全文
生物知识 Targeting mRNAs by Engineered Sequence-Specific RNase P Ribozymes The methods of using engineered RNase P catalytic RNA (termed as M1GS RNA) for in ... 04月17日 发表评论 阅读全文
生物知识 Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochon The MIA40 pathway is a novel import pathway in mitochondria specific for cysteine-... 04月17日 发表评论 阅读全文
生物知识 Nanodroplet Chemical Microarrays and Label-Free Assays The microarraying of chemicals or biomolecules on a glass surface allows for dense... 04月17日 发表评论 阅读全文
生物知识 Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Ph The minor inositol-containing membrane phospholipids, the phosphoinositides, play ... 04月17日 发表评论 阅读全文
生物知识 Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells The mitochondrial membrane potential is the dominant component of the proton-motiv... 04月17日 发表评论 阅读全文
生物知识 Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitoch The mitochondrial oxidative phosphorylation involves five multimeric complexes imb... 04月17日 发表评论 阅读全文
生物知识 Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells The mitogen-activated protein kinase (MAPK) signaling pathway is initiated by dive... 04月17日 发表评论 阅读全文
生物知识 Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum The model organismD. discoideumis well suited to investigate basic questions of mo... 04月17日 发表评论 阅读全文