The introduction of yeast artificial chromosomes (YACs) as cloning vectors in 1987...
生物知识
生物知识
生物知识
Invader Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation
The Invader� assay (Third Wave Technologies) is a homogeneous, isothermal DNA prob...
生物知识
PCR-SSP分析实验
原理根据决定某等位基因的碱基性质，设计3′端第一个碱基分别与各等位基因的特异性碱基相匹配的序列特异性引物，在PCR 反应过程中，只有引物3′端第一个碱基与决定特定等...
生物知识
Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications
The invention of real-time polymerase chair reaction (PCR) has revolutionized the ...
生物知识
Evaluating phenotype and genotype of drug-resistant strains in herpesviruses
The isolation of drug-resistant strains of herpesviruses, including Herpes Simplex...
生物知识
The Isolation of High Molecular Weight Eukaryotic DNA
The isolation of high molecular weight eukaryotic DNA in good yield is an importan...
生物知识
Isolation of Total Cellular DNA from Tissues and Cultured Cells
The isolation of high-quality cellular DNA is often a starting point for a variety...
生物知识
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the ...
生物知识
Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III
The knowledge of the distribution of ligand binding sites on extracellular domains...
生物知识
Biochemical determination of natural tumor-associated T-cell epitopes
The knowledge of tumor-associated T-cell epitopes is important for the understandi...
