生物知识 Design and Optimization of Sequence-Specific Hairpin Ribozymes The hairpin ribozyme belongs to a group of small catalytic RNAs that have been ext... 04月17日 发表评论 阅读全文
生物知识 改良 Leifson 氏染色法 一、简介细菌的鞭毛很纤细,其直径通常为 0.01 ~0.02 μm, 所以,除了很少数能形成鞭毛束(由多根鞭毛构成)的细圈可以用相差显微镜直接观察到鞭毛束的存在外,... 04月17日 发表评论 阅读全文
生物知识 Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences The hairpin ribozyme has been developed and designed to target and cleave efficien... 04月17日 发表评论 阅读全文
生物知识 Minimized Hammerhead Ribozymes The hammerhead ribozyme as engineered by Haseloff and Gerlach ( 1 ) consists of th... 04月17日 发表评论 阅读全文
生物知识 Enhancement of Ribozyme Function by RNA Binding Proteins The hammerhead ribozyme motif can be engineered to function as a sequence-specific... 04月17日 发表评论 阅读全文
生物知识 Crystallization and Final Stages of Purification The hard work has been done and the purification is “complete”—the target compound... 04月17日 发表评论 阅读全文
生物知识 Procedures for the Analysis and Purification of His-Tagged Proteins The heterologous expression of recombinant proteins is a valuable tool in the stud... 04月17日 发表评论 阅读全文
生物知识 High-resolution glycoprotein analysis using capillary electrophoresis The high-resolution separation achievable with capillary electrophoresis has been ... 04月17日 发表评论 阅读全文
生物知识 Single-Strand Conformation Polymorphism (SSCP) Analysis The identification of a large number of disease genes in recent years has led to a... 04月17日 发表评论 阅读全文
生物知识 Antibody Epitope Mapping Using Arrays of Synthetic Peptides The identification of antibody epitopes and their characterization at the amino ac... 04月17日 发表评论 阅读全文