1、试剂耗材的准备：（1）转膜缓冲液（48 mM Tris-HCl，39 mM 甘氨酸，0.037% SDS，20%甲醇）：（2）10 × 丽春红染液：（3）TBS...

ChIP是一项比较流行的研究转录因子（ transcription factor, TF）与启动子（promoter）相互结合的实验技术。由于ChIP采用甲醛固定活...

Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedure...

Contributor: Suprya JayadevDate: April 11,19961)Weigh out the appropriate amount o...

Reagents / SolutionsLysis Buffer:10ml 10% Sodium dodecyl sulphate (SDS)10ml Glycer...

This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The enz...

Preparation of cell lysates from E.coli by enzymatic lysisMaterialsChemicalslysosy...

This extraction procedure was developed by the Simmlaboratory and described in Rud...

SDS extraction followed by acetone precipitation – simple extraction protocol that...

Contributor: Suprya JayadevDate: September 21,1993Resin preparation:1)Measure out ...