生物知识 Directed Mutagenesis of Antibody Variable Domains The ability to mutate antibodies at the genetic level is a powerful tool in the an... 04月18日 发表评论 阅读全文
生物知识 Analysis of Urinary 8-oxo-7,8-dihydro-2-deoxyguanosine by Liquid ChromatographyTandem Mass Spectrome The ability to non-invasively monitor DNA oxidation and its repair has significant... 04月18日 发表评论 阅读全文
生物知识 Spectrophotometric Analysis of Nucleic Acids The ability to quantify nucleic acids accurately and rapidly is a prerequisite for... 04月18日 发表评论 阅读全文
生物知识 Systemic Delivery of Synthetic siRNAs The ability to regulate endogenous gene expression by cleaving mRNA is important i... 04月18日 发表评论 阅读全文
生物知识 The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling The ability to visualize the subcellular localization of proteins by labeling them... 04月18日 发表评论 阅读全文
生物知识 Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry The acylcarnitine profile is a diagnostic test for inherited disorders of fatty ac... 04月18日 发表评论 阅读全文
生物知识 Generation of Epitope-Tagged GPCRs The addition of one or more epitope tags to G-protein-coupled receptors (GPCRs) ha... 04月18日 发表评论 阅读全文
生物知识 Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products The advent of direct sequencing of polymerase chain reaction (PCR) products has pe... 04月18日 发表评论 阅读全文
生物知识 Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E The aim of this chapter is to provide a very practical set of instructions that wi... 04月18日 发表评论 阅读全文
生物知识 AP-PCR 方法 简介AP-PCR 方法,英文名是 arbitrarily primed PCR,是由 Williams 和 Welsh 同时在 1990 年建立起来的,由于它具有简... 04月18日 发表评论 阅读全文