生物知识 Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth Tetrahydromethanopterin (H4 MPT) is a tetrahydrofolate analog originally discovere... 04月18日 发表评论 阅读全文
生物知识 An efficient ligation method in the making of an in vitro virus for in vitro protein evolution The“in vitrovirus” is a molecular construct to perform evolutionary protein engine... 04月18日 发表评论 阅读全文
生物知识 Target-Site Selection for the 1023 DNAzyme The 10–23 DNAzyme is capable of cleaving RNA with high sequence specificity at sit... 04月18日 发表评论 阅读全文
生物知识 Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels The ability of two-dimensional electrophoresis (2-DE), based on the original metho... 04月18日 发表评论 阅读全文
生物知识 Restriction Endonuclease Digestion of DNA The ability to cleave DNA at specific sites is one of the cornerstones of today’s ... 04月18日 发表评论 阅读全文
生物知识 Site-Directed Mutagenesis The ability to create defined mutations and to generate precise fusions of protein... 04月18日 发表评论 阅读全文
生物知识 Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification The ability to directly manipulate the human genome to correct a disease-related m... 04月18日 发表评论 阅读全文
生物知识 Improved method for generating microarray probes using submicrogram amounts of total RNA The ability to identify disease-associated genes using microarray technology is de... 04月18日 发表评论 阅读全文
生物知识 Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template The ability to introduce specific changes into almost any given DNA sequence has r... 04月18日 发表评论 阅读全文
生物知识 Tobramycin Affinity Tag Purification of Spliceosomes The ability to isolate native ribonucleoprotein (RNP) particles is of fundamental ... 04月18日 发表评论 阅读全文