CARBONATE WESTERN TRANSFER SOLUTIONSDunn, Anal. Biochem. 1986: 1571.5L1.0L10mM NaH...

Culture bacteria1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl a...

Materials1.ｄｒｏｐ frozen 2x cycled tubulin in a polymerization-promoting buffer.Stor...

This protocol was written by Jean-Pierre Issa, based on Adams et al.* Kam-Wing Jai...

The discovery of this method allowed the application of laser desorption to protei...

Day 1-2Materials1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml.2.0.5 M KCl,50 mM HEPES,p...

This specific procedure was developed to assay the activity of the Lck kinase expr...

Northern BlotI. Electrophoresisclean gel box with NaOH and/or SDS, 2 hours to over...

ProcedureA: Preparation of the cell lysate1.Rinse a 60 mm culture dish of confluen...

Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repeti...