生物知识 Shotgun phage display Selection for bacterial receptins or other exported proteins Shotgun phage display cloning involves construction of libraries from randomly fra... 04月18日 发表评论 阅读全文
生物知识 General Consideration on Sialic Acid Chemistry Sialic acids, also known as neuraminic acids, are a family of negatively charged α... 04月18日 发表评论 阅读全文
生物知识 Mapping of Signaling Pathways by Functional Interaction Proteomics Signaling pathways transduce extracellular stimuli from the membrane to the nucleu... 04月18日 发表评论 阅读全文
生物知识 Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting Since the advent of hybridoma technology a quarter century ago, a large number of ... 04月18日 发表评论 阅读全文
生物知识 Production of Panels of Monoclonal Antibodies by the Hybridoma Method Since the first description by Kohler and Milstein (1 ), many variations on this m... 04月18日 发表评论 阅读全文
生物知识 Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis Since the first descriptions of electrophoresis in small diameter tubes in the 197... 04月18日 发表评论 阅读全文
生物知识 Inverse Polymerase Chain Reaction Since the first report on cDNA cloning in 1972 (1 ), this technology has been deve... 04月18日 发表评论 阅读全文
生物知识 Weak Affinity Chromatography Since the inception of affinity chromatography 30 years ago (1 ), it has developed... 04月18日 发表评论 阅读全文
生物知识 Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis Since the introduction of solid-phase peptide synthesis by Merrifield (1 ) nearly ... 04月18日 发表评论 阅读全文
生物知识 Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins Since the pioneering publication of first paper on application of capillary electr... 04月18日 发表评论 阅读全文