生物知识 半巢式 PCR 简介半巢式 PCR 是利用三条引物进行两次 PCR 扩增的方法,与巢式 PCR 原理相同,只是在第 2 轮 PCR 反应中使用的引物有 1 条为第 1 轮 PCR ... 04月18日 发表评论 阅读全文
生物知识 High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment Several formats—small, standard, and large—can be employed to separate proteins by... 04月18日 发表评论 阅读全文
生物知识 Ubiquitin Chain Synthesis Several important signaling processes depend on the tagging of cellular proteins w... 04月18日 发表评论 阅读全文
生物知识 Formation and Characterization of Hepatocyte Spheroids Several investigators have demonstrated that freshly harvested hepatocytes self-as... 04月18日 发表评论 阅读全文
生物知识 Dealing with different methods for Kluyveromyces lactis -galactosidase purification Several micro-scale chromatography-based procedures for purification of the β-gala... 04月18日 发表评论 阅读全文
生物知识 Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells Several pathological and disease conditions can alter the mechanical properties of... 04月18日 发表评论 阅读全文
生物知识 Nonradioactive Northern and Southern Analyses From Plant Samples Several specific problems are encountered when nonradioactive detection methods ar... 04月18日 发表评论 阅读全文
生物知识 Oscillating Probe for Dual Detection of 5’PO4 and 5’OH DNA Breaks in Tissue Sections Several types of DNA cuts are used as markers of apoptosis for detection of apopto... 04月18日 发表评论 阅读全文
生物知识 Cryopreservation of Shoot Tips and Meristems Shoot-tip meristem cryopreservation methodologies are reported for the complementa... 04月18日 发表评论 阅读全文
生物知识 Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo Short-range fluorescence quenching has proven to be an effective method to detect ... 04月18日 发表评论 阅读全文