材料与仪器步骤一、材料1. 缓冲液、溶液和试剂去除 RNA 酶的双蒸水10XRT-PCR 缓冲液（去除 RNA 酶的双蒸水，100 mmol/LTris-HCl、p...
生物知识
生物知识
生物知识
Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Des
Ribozymes first bind their target via complementary sequences like a conventional ...
生物知识
Quantitation of Ribozyme Target Abundance by QCPCR
Ribozymes show promise as therapeutic agents for the downregulation of specific ta...
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Preparation of RNA Dot Blots
RNA dot hybridizations were first described by Kafatos et al. (1 ). These hybridiz...
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Preparation of RNA Gel Blots
RNA gel blots (often referred to as Northern gel blots) are frequently used in the...
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Nonradioactive Methods for the Detection of RNA-Protein Interaction
RNA in biological systems is associated with proteins. Recent work in eukaryotes h...
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Quantification of siRNAs In Vitro and In Vivo
RNA interference (RNAi) is a regulatory mechanism of eukaryotic cells that uses sm...
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A rapid and sensitive assay for quantification of siRNA efficiency and specificity
RNA Interference has rapidly emerged as an efficient procedure for knocking down g...
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Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
RNA polymerase (RNAP) is a DNA-dependent motor protein that links ribonucleotide p...
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Genome Wide Full-Length Transcript Analysis Using 5 and 3 Paired-End-Tag Next Generation Sequencing
RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcrip...
