生物知识 TRIzol RNA提取方法 (一)试剂准备1.TRIzol试剂。2.氯仿3.异丙醇4.75%乙醇(DEPC H2O配制)5.DEPC H2O(二)操作步骤1. 样品处理:(1)培养细胞:收获细... 04月18日 发表评论 阅读全文
生物知识 生物氧化复习笔记 1、 物质在体内氧化和体外氧化有什么异同?体内氧化即是生物氧化。它是在细胞内的由酶催化的氧化反应,几乎每步反应都由酶来催化进行。不需要高温,也步需要强碱及强氧化剂的... 04月18日 发表评论 阅读全文
生物知识 Chemical Functionalization and Bioconjugation Strategies for Atomic Force Microscope Cantilevers Over the last decade, scanning probe microscopy (SPM) techniques, such as atomic f... 04月18日 发表评论 阅读全文
生物知识 Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System Over the last two decades, the use of eukaryotic cells for expression of recombina... 04月18日 发表评论 阅读全文
生物知识 Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice Over the past 25 years, genetically engineered mouse models have become an integra... 04月18日 发表评论 阅读全文
生物知识 7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophore Over the past decade, fluorescent end-labeling of DNA fragments has evolved into t... 04月18日 发表评论 阅读全文
生物知识 Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides Over the past decade, numerous nuclear and cytosolic glycoproteins have been shown... 04月18日 发表评论 阅读全文
生物知识 Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis Over the past few decades, carbohydrates (glycans) have received growing attention... 04月18日 发表评论 阅读全文
生物知识 Single-Molecule Protein Unfolding and Refolding Using Atomic Force Microscopy Over the past few years, atomic force microscopy (AFM) became a prominent tool to ... 04月18日 发表评论 阅读全文
生物知识 Microwave-Assisted Formalin Fixation of Fresh Tissue: A Comparative Study Over the past two decades, there has been a growing interest in reducing the turna... 04月18日 发表评论 阅读全文