猪肺泡巨噬细胞(3D4/21)猪肺泡巨噬细胞(3D4/21)介绍：母细胞3D4来源于pSV3neo质粒转染猪原代肺泡巨噬细胞得到的。该克隆通过G418筛选得到。猪肺...

Studying Protein Interactions by Far-Western BlottingFar-Western blotting was orig...

Marian Price-Carter, 9/7/00Day 1 : Start overnight cultures in assay medium.Negati...

1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is us...

(All steps with agitation, unless done O/N at 4℃)►Rinse membrane once in TBS-T►Blo...

Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH f...

Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Protein...

Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml fo...

Research Tools: Synthetic Biology and Protein EngineeringResearch Goals: (1)Improv...

Blotting with BSA1. Blot in 5% mile/ 1X PBS T (or 1X TBST) --60'/Rt2. Wash 2X...