NIH3T3细胞中本身是没有TK的，1981年Scolnick利用lambda载体把胸腺嘧啶激酶（TK）转入NIH3T3细胞，构建成NIH3T3 TK细胞，所以细胞...
生物知识
生物知识
生物知识
Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models
The mainstay of clinical antineoplastic chemotherapy is multiagent combinations, m...
生物知识
Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Us
Real-time quantitative polymerase chain reaction (QPCR) using the Roche LightCycle...
生物知识
Direct tissue explant method for derivation and study of extravillous trophoblas
From early post-implantation stages to approximately 18 weeks' gestation, cyt...
生物知识
Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
Ovarian cancer remains a major health problem in the United States and most Wester...
生物知识
Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques
The molecular analysis of human cancer is complicated by the difficulty in obtaini...
生物知识
Flow Cytometric Analysis of p53-Induced Apoptosis
The p53 protein is a key player in the cellular response to stress conditions. Act...
生物知识
Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
Growth factors are polypeptides that induce cell mitogenicity, and thus play an im...
生物知识
Analyzing the Spindle Checkpoint in Yeast and Frogs
The spindle checkpoint is an evolutionarily conserved regulatory mechanism that en...
生物知识
DNA-Mediated Gene Transfer
Transfection is the process by which exogenous DNA is transferred into eukaryotic ...
