Materials DEPC H2 O DEPC 0.1% (v/v) q.s. de-ioinized H2O 37ºC x1 hr, or r.t. overn...
生物知识
生物知识
生物知识
SDS-polyacrylamide gel electrophoresis
SOLUTIONS30% acrylamide mix29% (w/v) acrylamide1% (w/v) N,N''- methylene...
生物知识
Two Dimensional Gel Electrophoretic Analysis for the Human Plasma
Overview This protocol is a detail description of the laboratory procedure in perf...
生物知识
Amino acid composition
There has been a recent revival of interest in the use of AA composition for the i...
生物知识
人结直肠癌氟尿嘧啶耐药株（HCT-15/FU）
人结直肠癌氟尿嘧啶耐药株（HCT-15/FU）人结直肠癌氟尿嘧啶耐药株（HCT-15/FU）介绍：这是一株耐药细胞株。人结直肠癌氟尿嘧啶耐药株（HCT-15/FU）...
生物知识
Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Assemble each gel sandwich by stacking, in order, the notched aluminum plate, two ...
生物知识
BASIC-NATIVE GEL Protocol
Stock Solutions1) 1.5M TrisHCl pH 8.9 - Keep RT.2) 30% Acrylamide 0.8% Methylene b...
生物知识
Analysis of Proteins using Small Format 2D Gel Electrophoresis
Preparation of protein samplesIntracellular virus proteinsThe following method has...
生物知识
广州赛诚生物EMSA核心实验技术：实验流程
一、探针 的标记1.如下设置探针标记的反应体系：(1)待标记探针(1.75 pmol/微升)：2微升。(2)T4 Polynucleotide Kinase Buf...
生物知识
DNA的酶切技术
重组DNA技术工具酶一、实验目的1.通过对DNA的酶切，学会设计构建体外重组DNA 分子;2.根据目的基因合理选择载体与限制性内切酶 ;3.掌握DNA的酶切技术。二...
