1.PCR反应的最适条件1．1 TaqDNA聚合酶在早期进行的PCR反应中，使用的是大肠杆菌DNA聚合酶1的大片段，即Klenow片段。也曾有人用噬菌体T4 DNA...

早在1958年科学家分离出DNA聚合酶（ polymerase）后，就曾设想利用DNA聚合酶扩增大量DNA片段。但当时由于DNA测序较难，没有核苷酸合成技术及耐热性...

1.DMSO and glycerolRecommended by a number of authors to improve amplification eff...

General AdvicePCR allows the production of more than 10 million copies of a target...

进化遗传学具有两个并列的研究方向：系统发育的重建和种群分析。自1962年， Zuckerkandl和Pauling提出蛋白质序列和基因序列的比较可以象分子种一样用于...

The identification of novel members of gene families by PCR using degenerate prime...

Recommended Reagent Concentrations:Primers: 0.2 - 1.0 uM 　　Nucleotides: 50 - 200 u...

Nature of the ｉｎｓｅｒｔThe cloning of PCR-amplified fragments into a linear vector is...

Procedure1. Set up the following PCR tube:5 μl of 10X Taq Buffer5 μl of 25 mM MgCl...

Hi everyone, I'll start doing rt-pcr using sybr green chemistry and already a...