Liquid chromatography (LC) can be combined with matrix-assisted laser-desorption i...
生物知识
生物知识
生物知识
Measurements of White Adipose Tissue Metabolism by Microdialysis Technique
The microdialysis method was introduced 25 yr ago to measure neurotransmitter conc...
生物知识
MTS检测细胞生长
1． 培养 IL-2 依赖细胞株 CTLL-2 细胞或 HT-2 细胞（检测 IL-2 ），或者 IL-3 依赖细胞株 FDC-P1 细胞或 FL5.12 细胞（检...
生物知识
Classical Human Epidermal Keratinocyte Cell Culture
It has been more than 30 years since the serial cultivation of human keratinocytes...
生物知识
Assays to Measure Stress-Activated MAPK Activity
Mitogen-activated protein kinases (MAPKs) are activated by a wide variety of cellu...
生物知识
MudPIT Analysis: Application to Human Heart Tissue
Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been use...
生物知识
肽酰转移酶(peptidyl transferase)
肽酰转移酶是催化肽键形成的酶。在蛋白质合成过程中，它催化核糖体A位tRNA上末端氨基酸的氨基与P位肽酰-tRNA上氨基酸的羧基间形成肽键。其结果,使A位的氨酰-tR...
生物知识
Simplified Calcium Phosphate Coprecipitation
Materials2x HEPES: 8 g NaCl, 0.105g NaHPO4 , 6.5 g HEPES, H2O to 500 mL, pH 6.95 t...
生物知识
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
The use of affinity tags and especially histidine tags (His-tags) has become wides...
生物知识
光系统(photosystem)
进行光吸收的功能单位称为光系统, 是由叶绿素、类胡萝卜素、脂和蛋白质组成的复合物。每一个光系统含有两个主要成分∶捕光复合物(light -harvesting co...
