Phospholipase D (PLD) hydrolyzes phosphatidylcholine to produce the membraneassoci...
生物知识
生物知识
生物知识
Online Tools for Predicting Integral Membrane Proteins
We identify and describe a set of tools readily available for integral membrane pr...
生物知识
Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections
Immunohistochemistry is a powerful technique to localize proteins in tissues and c...
生物知识
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
Description This protocol is for use with the D cyclins and employs 488 nm argon l...
生物知识
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluoresc...
生物知识
Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy
RNA interference or RNAi-based gene therapy for the treatment of HIV-1 infection h...
生物知识
Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
Oxidative stress has been shown to be one of the mechanisms involved in a number o...
生物知识
细胞生长检测3：XTT检测法
XTT检测法用 XTT 代替 MTT 可省去溶解还原产物结晶的步骤， XTT 可以被活细胞中的代谢酶还原成黄色水溶性的代谢产物（ formazan ）。代谢产物在 ...
生物知识
Dorsal Root Ganglion Neuron Culture from Adult Rats
Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neu...
生物知识
II型内含子(group II introns)
主要存在于线粒体中的一类内含子，它的剪接位点类似于核编码结构基因的内含子,并同样遵从GT--AG规律。剪接机理同核内含子的剪接相似,也要形成一个套索的中间体,通过形...
