生物知识 电子自旋共振谱技术(electron spin-resonance spectroscopy,ESR) 证明膜脂流动性的一种方法。在该技术中将一个含有不配对的电子基团(通常是硝基氧基团)加到磷脂的脂肪酸尾端,这就是所谓的自旋标记(spin-label )。当将这种脂暴... 04月27日 发表评论 阅读全文
生物知识 Purification of Fibronectin from Human Plasma Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble f... 04月27日 发表评论 阅读全文
生物知识 Immobilization of Enzymes by Bioaffinity Layering Bioaffinity immobilization exploits the affinity of the enzyme to a macro-(affinit... 04月27日 发表评论 阅读全文
生物知识 Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation The ERK signaling cascade is composed of several protein kinases that sequentially... 04月27日 发表评论 阅读全文
生物知识 Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber) Overview This assay uses a simple apparatus to study chemotaxis of leukocytes or o... 04月27日 发表评论 阅读全文
生物知识 Generation of Human-Induced Pluripotent Stem Cells by Lentiviral Transduction Human somatic cells can be reprogrammed to the pluripotent state to become human-i... 04月27日 发表评论 阅读全文
生物知识 CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins We present a newly developed method for fixing RNA-protein complexesin situin livi... 04月27日 发表评论 阅读全文
生物知识 Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies In this chapter, two techniques for the analysis of transport through the nuclear ... 04月27日 发表评论 阅读全文
生物知识 Analysis of Meiotic Recombination Products from Human Sperm Traditional methods for surveying meiotic recombination in humans are limited to p... 04月27日 发表评论 阅读全文
生物知识 Cryopreservation of Cell Lines Aim The protocol below describes the use of passive methods involving an electric ... 04月27日 发表评论 阅读全文