生物知识 Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaio MATERIALST-cell:CD2IgG2b, 01171DB-cell:B220 (CD45R)IgG2a, 01121ACD3IgG2b, 28001DMo... 04月27日 发表评论 阅读全文
生物知识 Multilocus Sequence Typing Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach ... 04月27日 发表评论 阅读全文
生物知识 Polymer Microarrays for Cellular High-Content Screening Polymer microarrays as platforms for cell-based assays are presented, offering a u... 04月27日 发表评论 阅读全文
生物知识 An Efficient Transfection Method for Mouse Embryonic Stem Cells Embryonic stem (ES) cells are an important source of stem cells in tissue engineer... 04月27日 发表评论 阅读全文
生物知识 Subculture of Semi-Adherent Cell Lines Aim Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a propo... 04月27日 发表评论 阅读全文
生物知识 Phycoerythrin conjugation protocol Phycoerythrin conjugation protocolDavid's method modified from references (2)... 04月27日 发表评论 阅读全文
生物知识 初代组织块培养法 1. 剪切:把组织小块置于小烧杯或青霉素小瓶中,用 Hanks 液漂洗二三次以去掉表面血污,吸静 Hanks 液,用眼科剪反复剪切 1mm3 块为止。2. 摆布:用... 04月27日 发表评论 阅读全文
生物知识 Liposome-Based Vaccines Here, we report methods of preparation of liposome vaccine formulations for the en... 04月27日 发表评论 阅读全文
生物知识 Mechanism-Based Profiling of MMPs The recognition that the successful clinical use of MMP inhibitors will require qu... 04月27日 发表评论 阅读全文
生物知识 Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Fo Epstein Barr virus (EBV) can be used to transform human B-lymphocytes to derive po... 04月27日 发表评论 阅读全文