生物知识 Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae Free-living amoebae (FLA) are unicellular, eukaryotic protozoa found in virtually ... 04月27日 发表评论 阅读全文
生物知识 Flow Cytometry Assay to Measure Internalization of GPCR’s Flow Cytometry Assayto Measure Internalization of GPCR'scontributed by Patric... 04月27日 发表评论 阅读全文
生物知识 Xenopus Egg Extracts: A Model System to Study Proprotein Convertases TheXenopusegg extract translation system has proved an ideal tool with which to st... 04月27日 发表评论 阅读全文
生物知识 Chromosome Spreads with Centromere Staining in Mouse Oocytes This chapter describes a technique for performing chromosome spreads from mouse oo... 04月27日 发表评论 阅读全文
生物知识 Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Sa Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its product... 04月27日 发表评论 阅读全文
生物知识 加温除菌法 一、简介细胞受各种霉菌、细菌和支原体污染后,一般都较难排除或杀灭,其中以支原体更难排除,因此从预防着眼为上。二、操作方法加温除菌法三、材料与仪器细胞步骤根据支原体对... 04月27日 发表评论 阅读全文
生物知识 alamarBlue的用法 贴壁细胞和非贴壁细胞都适用。所有操作在无菌条件下进行。避免污染细胞,因为微生物污染也会还原alamarBlueTM ,培养基中应添加抗生素如青霉素G(1U/ml)、... 04月27日 发表评论 阅读全文
生物知识 Protamine Extraction and Analysis of Human Sperm Protamine 1/Protamine 2 Ratio Using Acid Gel Electr Protamines, sperm-specific nuclear proteins, are essential for sperm chromatin con... 04月27日 发表评论 阅读全文
生物知识 Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectromet The following chapter describes a method to measure argatroban in plasma samples u... 04月27日 发表评论 阅读全文
生物知识 Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or St A number of endogenously biotinylated proteins are found in both cytosol and mitoc... 04月27日 发表评论 阅读全文