刺糖多孢菌一、菌种简介1、菌种名称：刺糖多孢菌Saccharopolyspora spinosa2、菌种编号：BMZ0284453、其它中心编号：=ATCC 494...
生物知识
生物知识
生物知识
脱氮假单胞菌
脱氮假单胞菌一、菌种简介1、菌种名称：脱氮假单胞菌Pseudomonas sp.2、菌种编号：B814423、其它中心编号：ATCC138674、具体用途：研究、教...
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PCR应用中存在的问题
肖生祥 （西安医科大学第二临床医学院皮肤科，710004） 开展 PCR 应具有一定的条件。需要一个正规的 pCR 实验室，采集标本、核酸提取、扩增及产物分析应在...
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Standard RT-PCR
RT-PCR or reverse transcription PCR refers to PCR that uses product of an RT react...
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An Economic PCR Enhancer for GC-Rich PCR Templates
Since PCR is often problematic on GC-rich templates, we have evaluated different P...
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Ethanol precipitation method for purifying PCR products
1. For each PCR reaction (50 µL) prepare a 1.5mL microcentrifuge tube containing t...
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Polymerase Chain Reaction (PCR) cont.
Polymerase Chain Reaction (PCR) cont.Choice of Polymerases for PCROne of the impor...
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Blunt end cloning of PCR products
End repair:Add 5-10 units of T4 DNApol and incubate at 37C for 5 minutes. Make sol...
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Single Primer (Semi-Random) PCR
Single Primer ("Semi-Random") PCRJuly 26, 2000 ECKDescriptionSingle prim...
