生物知识 Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and... 04月27日 发表评论 阅读全文
生物知识 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold The study of neuronal connectivity requires the ability to trace axons from the ne... 04月27日 发表评论 阅读全文
生物知识 Application of a Fusiogenic Peptide GALA for Intracellular Delivery To enhance the cytosolic delivery of therapeutic drugs and genes, pH-sensitive and... 04月27日 发表评论 阅读全文
生物知识 ES and TS cell freezing/thawing ES and TS cell freezing/thawingNeeded:ES cell freezing medium (2x)2x ES cell freez... 04月27日 发表评论 阅读全文
生物知识 Covalent Modification of G-Proteins by Affinity Labeling The activation of heterotrimeric G-proteins is tightly regulated by the exchange o... 04月27日 发表评论 阅读全文
生物知识 Generation and Hepatic Differentiation of Human iPS Cells A method for the generation of human induced pluripotent stem (iPS) cells was esta... 04月27日 发表评论 阅读全文
生物知识 Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase Membrane enzymes may be peripheral or integral proteins. The attachment of periphe... 04月27日 发表评论 阅读全文
生物知识 Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free C This chapter describes the procedures in order to do immunofluorescence (IF) micro... 04月27日 发表评论 阅读全文
生物知识 Simultaneous analysis of DNA content and surface Simultaneous analysis of DNA content and surface immunophenotype using gentle etha... 04月27日 发表评论 阅读全文
生物知识 Lentivirus的包装和感染 相关专题 1 转染前一天将293T细胞铺于10cm板,当细胞密度达到80%左右即可进行PEI转染。 2将下列DNA加入一个已装有480ul 1* HBS( PH 7... 04月27日 发表评论 阅读全文