重组节是同源染色体配对联会复合体中的球形、椭圆型或棒状的结节, 直径约为90nm, 是有蛋白质装配成的小体, 结构不清楚。重组节中含有催化遗传重组的酶类，因此推测某...
生物知识
生物知识
生物知识
Determination of MiRNA Targets in Skeletal Muscle Cells
MicroRNAs (miRNAs) are a class of small ∼22 nucleotide noncoding RNAs which regula...
生物知识
Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Cult
The endothelium residing in different vascular beds displays high-degree phenotypi...
生物知识
Origin and Progress of Nuclear Transfer in Nonmammalian Animals
This chapter traces the orgin and progress of nuclear transfer that later became t...
生物知识
Multiplexing Cell Viability Assays
Today, obtaining mechanistic insights into biological, toxicological, and patholog...
生物知识
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase C
Ligation-mediated polymerase chain reaction (LM-PCR) is used for high-resolution g...
生物知识
Lentivirus-Expressed siRNA Vectors Against Alzheimer Disease
Amyloid precursor protein (APP) has been implicated in the pathogenesis of Alzheim...
生物知识
Apoptosis TUNEL assay (Paraffin Sections)
Protocol for Paraffin Sections: Dewax paraffin sections:Incubate slides, 55°C, 30 ...
生物知识
限制点（restriction point）
限制点是哺乳动物细胞周期G1期控制进入S期的调节点，相当于酵母的START点。主要是通过体外细胞培养实验发现的。哺乳动物细胞体外培养时，需要添加多肽生长因子促进细胞...
生物知识
Detection of Mycoplasma by Culture
Aim Detection of mycoplasma by culture is the reference method of detection and ha...
