NH4Ac and EtOH precipitation of DNAAdd NH4Ac (10M stock or solid) to the sample fo...

Procedure for 100 ~ 300 mg of tissue.1. Collect fresh tissue, coleoptiles or small...

Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM EDT...

Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragments ...

Phenol (removes protein)1、add equal volume of Phenol (= tris-saturated Phenol-Chlo...

Glass wool method:Run TAE agarose gel and cut the appropriate band out with a clea...

1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi...

Preparation of Sonicated Salmon Sperm DNAProcedure:1) Use Pharmacia #27-4564-01. W...

1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampici...

ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A pe...