3'RACE PCR 3'RapidAmplification ofcDNAEnds (RACE) PCRThis technique is u...
生物知识
生物知识
生物知识
DIETHYL PYROCARBONATE (DEPC) TREATMENT OF AQUEOUS SOLUTIONS
Diethyl pyrocarbonate derivitizes histidine residues and is therefore an effective...
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Primer Extension analysis of total yeast RNA
Primer Extension analysis of total yeast RNA1. Mix the following in a 1.5 ml eppen...
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In vitro RNA synthesis from plasmid-borne sequences under th
N.B: Gloves should be worn at all times during preparation of in vitro RNA. All so...
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RNA purification — hot phenol
Hot phenol may also be used to remove DNA.1. Add equal volume of TE-saturated phen...
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S1analysis of yeast mRNA using oligo nucleotide probes
Steve Hahn. last modified Sat, Oct 17, 1998Mix the following in an 0.5 ml eppendor...
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Use of Internaland External Standardsor Reference RNAs for A
IntroductionNorthern blotting, nuclease protection assays and RT-PCR are frequentl...
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Protocol for First-strand cDNA Synth
Prepare in a sterile tube:template RNA: total RNA 0.1-5µg or poly(A)+ mRNA 10ng-0....
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PCR/SSP技术
一、血清学分型技术1. HLA-Ⅰ类抗原的检测HLA-A、B、C抗原型别鉴定均借助微量淋巴细胞毒试验（microlymphocytotoxicitytest）或称补...
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PCR-SSP结果分析
PCR-SSP结果分析采用SSP进行PCR扩增后，相应的PCR产物是否出现，是HLA分型结果的判断的依据，相应SSP扩增产物出现，表示基因组中存在与特异性引物（即S...
