The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are usi...
生物知识
生物知识
生物知识
Invitro transcription
The following protocol is for MEGAscript II Kit (Ambion).1. PCR amplify the DNA te...
生物知识
RNAi 实验介绍
1. RNAi 介绍 RNA 干扰（RNAi:RNA interference）是由诺贝尔生理学/医学奖得主 Andrew Z. Fire 和 Craig C. M...
生物知识
cDNA的制备与检测
[实验原理] 总RNA，经寡聚(d丁)纤维素亲和层析柱分离出mRNA，以寡聚(d丁)为引物，经反转录合成双链cDNA。先用反转录酶合成第一条cDNA链；经碱降解...
生物知识
cDNA合成技术
Promega公司的RibocloneR M-MLV(H -) cDNA合成系统采用M-MLV反转录酶的RNase H缺失突变株取代AMV反转录酶，使合成的cDNA...
生物知识
96-well RNA In Situ Hybridization Protocol
The RNA in situ procedure described below is based on the protocol developed by Ta...
生物知识
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (C
Prepare in a sterile tube:template RNA:total RNA 0.1-5µgor poly(A)+ mRNA 10ng-0.5µ...
生物知识
cDNA Synthesis from MOLT-4 Cells
First strand of cDNA synthsis1.Add 2 μl of Not I primer-adapter to a sterile 1.5 m...
生物知识
ESTs from Phage Plaques
The Queensland Institute for Medical Research (QIMR) lab's method for automat...
生物知识
RNA的提取
一、准备试剂：氯仿，异丙醇，75℅乙醇，无RNase的水或0.5℅SDS（溶液均需用DEPC处理过的水配制）。二、操作步骤：1. 匀浆处理：a. 组织 将组织在液氮...
