1. Collected animals can be stored as frozen pellets at -70 degrees. Alternatively...
生物知识
生物知识
生物知识
Decontamination and inhibition of ribonucleases
Decontamination and inhibition of ribonucleasesIt is common knowledge in most labo...
生物知识
Collection of Tissue for RNA Analysis
Handling RNA:Ribonucleases (RNases) are very stable and active enzymes that degrad...
生物知识
Northern Blotting (Howell Lab)
RNA isolationHarvest cells when they are about 80-85% confluent. Stationary phase ...
生物知识
[Nature] RNAi研究专题
Cells utilize RNA interference (RNAi) to regulate protein expression in many conte...
生物知识
Hiro Hirai PRINS Protocol
Hiro Hirai 's PRINS ProtocolOverviewThe PRINS (Primedin situ) technique uses ...
生物知识
E.coli Total RNA Labeling Protocol for Spotted Microarray
Note: Start with 20 mg of total RNA for each labeling reaction. All solutions that...
生物知识
RNAi-制备siRNAs的方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链RN...
生物知识
Northern Blotting: Efficient RNA Staining and Transfer
(original protocol by R. M. Fourney, S. Miyakoshi, R. S. Day III, and M. C. Peters...
生物知识
Protein-DNA Telomere FISH
Protein-DNA Telomere FISHFIX AND PROTEIN STRAIN:Fix cells in 4% paraformaldhyde (d...
