One of the most problematic steps in RNA isolation is the first step - thorough ly...
生物知识
生物知识
生物知识
What is your Protocol for RNAi on Cell Cultures
6-Well PlatesBathingPrepare dsRNA suspended in water.We use ~500 bp dsRNA.Add ~10-...
生物知识
Digoxigenin-UTP RNA labeling
Digoxigenin-UTP RNA labelingDigoxigenin-UTP RNA labeling 1. Add the following to a...
生物知识
PREPARATION OF STOCK SOLUTIONS FOR FISH
PREPARATION OF STOCK SOLUTIONS FOR FISHnote: use for all preparations deionised, b...
生物知识
Fluorometric Quantitation of RNA or DNA
Materials DNA: Höchst 33258 (High range solution): (Invitrogen H1398 or Sigma B115...
生物知识
Northern Blot实验方法
[仪器、试剂、材料] （一）仪器 恒温水浴箱，电泳仪，凝胶成像系统，真空转移仪，真空泵，UV 交联仪，杂交炉，恒温摇床，脱色摇床，漩涡振荡器，分光光度计，微量移液器...
生物知识
The Basics: RNase Control
To the average molecular biologist working with RNA, preventing, detecting and eli...
生物知识
Northern Blot: Glyoxal/DMSO
Northern Blot: Glyoxal/DMSOAdapted from Molecular Cloning (Maniatis), Current Prot...
生物知识
Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1: Make complex...
生物知识
Concentrating poly(A)+ mRNA
Concentrating poly(A)+ mRNASource:Protocol OnlineAbstract:Simple precipitation met...
