生物知识 Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis The skin as a surface organ is uniquely accessible for whole embryo/foetal analyse... 05月01日 发表评论 阅读全文
生物知识 Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies ar... 05月01日 发表评论 阅读全文
生物知识 Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation Dendritic cells (DCs) are the main regulators of adaptive immune responses and con... 05月01日 发表评论 阅读全文
生物知识 In Vitro Human Skin Segmentation and Drug ConcentrationSkin Depth Profiles Highly optimized methods for skin segmentation are provided using tape stripping i... 05月01日 发表评论 阅读全文
生物知识 The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases There are two key methods which yield information about the cellular function of a... 05月01日 发表评论 阅读全文
生物知识 CHO Centrosome Prep CHO Centrosome Prep:Arshad Desai4/94Cells:We grow our CHOs with MEM[[alpha]] (with... 05月01日 发表评论 阅读全文
生物知识 Heme Oxygenase Activity Current Methods and Applications The heme oxygenase enzymes (HO-1 and HO-2) oxidize heme to biliver-din-IXα (BVIXα)... 05月01日 发表评论 阅读全文
生物知识 Real-Time BRET Assays to Measure G Protein/Effector Interactions Advances in imaging assays based on resonance energy transfer (RET) have made it p... 05月01日 发表评论 阅读全文
生物知识 In Situ Hybridization of Suprachiasmatic Nucleus Slices The progress in the understanding of the molecular machinery of mammalian circadia... 05月01日 发表评论 阅读全文
生物知识 Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Bloo This chapter describes in vitro methods for studying the Blood-Brain barrier. Thes... 05月01日 发表评论 阅读全文