上皮细胞培养1）表皮细胞培养1． 取材：取外科植皮或手术残余皮肤小块，以角化层薄者为佳，早产流产儿皮肤更好，切成 0.5 ～ 1 平方厘米小块。2． EDTA 处理...
生物知识
生物知识
生物知识
The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic -Cell
Embryonic stem (ES) cells represent an attractive tool not only for the study of t...
生物知识
Protein (Western) Blotting with Immunodetection
Protein blotting simply involves transferring separated protein bands from an acry...
生物知识
Culturing Mouse Embryonic Fibroblasts
MaterialsTrypsin (Gibco 25200-023) 3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS...
生物知识
Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways
Reverse genetic methods, such as homologous gene targeting, have greatly contribut...
生物知识
Lipid Profiling Using Two-Dimensional Heteronuclear Single Quantum Coherence NMR
The use of NMR spectroscopy in lipid research has been traditionally reserved for ...
生物知识
High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
Cytochromes P450 are a superfamily of enzymes that catalyze the monoxygenation of ...
生物知识
Retroviral Transduction of Murine Primary T Lymphocytes
In comparison to human T cells, efficient retroviral gene transfer and subsequent ...
生物知识
Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Bi
There are a number of disadvantages with conventional tissue immunohistochemistry ...
生物知识
Analysis of Fiber-Type Differences in Reporter Gene Expression of -Gal Transgenic Muscle
β-galactosidase (β-gal) is among the most frequently used markers for studying a w...
