生物知识 Recording Ion Channels in Isolated, Split-Opened Tubules Ion channels play key roles in physiology. They function as protein transducers ab... 05月02日 发表评论 阅读全文
生物知识 PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D We describein situhybridization protocols using peptide nucleic acid (PNA) as a pr... 05月02日 发表评论 阅读全文
生物知识 Sources and Derivation of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner ce... 05月02日 发表评论 阅读全文
生物知识 Methods for Analysis of Ligand-Induced RNA Conformational Changes Encoded within many RNA sequences is the requisite information for folding of intr... 05月02日 发表评论 阅读全文
生物知识 Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation DNA methylation and histone modifications are key regulatory mechanisms in cellula... 05月02日 发表评论 阅读全文
生物知识 Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot Human embryonic stem cells (hESCs) represent a promising source of cells for moder... 05月02日 发表评论 阅读全文
生物知识 Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging The formation of new synapses within neuronal circuits is considered a primary mec... 05月02日 发表评论 阅读全文
生物知识 Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep Radioiodinated transforming growth factors ( TGF-βs) are critical reagents for fun... 05月02日 发表评论 阅读全文
生物知识 Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis An electrophoretic mobility shift assay (EMSA) is a common and invaluable techniqu... 05月02日 发表评论 阅读全文
生物知识 Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors During the last few years, poly(ADP-ribose)polymerase (PARP) proteins became a ver... 05月02日 发表评论 阅读全文