生物知识 Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET Drug “ligands” that bind G protein-coupled receptors (GPCRs) can either stimulate,... 05月02日 发表评论 阅读全文
生物知识 Characterization of B-Cell Maturation in the Peripheral Immune System In the periphery different populations of B cells can be identified, corresponding... 05月02日 发表评论 阅读全文
生物知识 Cystic Fibrosis Airway Epithelial Cell Culture Cystic fibrosis (CF) is the most frequent (incidence around l/2500 live births) ge... 05月02日 发表评论 阅读全文
生物知识 Generation and Use of Epitope-Tagged Receptors Epitope tagging of a receptor involves introducing a defined amino acid sequence, ... 05月02日 发表评论 阅读全文
生物知识 Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells The introduction of restriction endonucleases into mammalian cells in culture prov... 05月02日 发表评论 阅读全文
生物知识 Cryopreservation of Shoot-Tips and Meristems The ability to regenerate whole plants from cryopreserved, meristematic shoot tiss... 05月02日 发表评论 阅读全文
生物知识 Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Head and neck reconstruction transplants often require a bony structure but also t... 05月02日 发表评论 阅读全文
生物知识 High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophi Preservation ofTetrahymena thermophilabasal body ultrastructure for visualization ... 05月02日 发表评论 阅读全文
生物知识 Assessing PCP in the Cochlea of Mammalian Ciliopathy Models The increased availability of mouse models of human genetic ciliary diseases has l... 05月02日 发表评论 阅读全文
生物知识 Multiple Site-Directed Mutagenesis Site-directed mutagenesis techniques allow selective engineering of gene sequences... 05月02日 发表评论 阅读全文