生物知识 COPⅠ被膜小泡(COPⅠcoated vesicle) 由外被蛋白Ⅰ(coat protein Ⅰ, COP Ⅰ )包裹的小泡。主要介导蛋白质从高尔基体运回内质网,包括从反面高尔基体运向顺面高尔基体,以及将蛋白质从反面高... 05月03日 发表评论 阅读全文
生物知识 Experimental Control and Characterization of Autophagy in Drosophila Insects such as the fruit flyDrosophila melanogaster, which fundamentally reorgani... 05月03日 发表评论 阅读全文
生物知识 Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs A common approach to the study of gene function inXenopusembryos is to ectopically... 05月03日 发表评论 阅读全文
生物知识 Utility of Animal Models for Human Embryo Culture: Nonhuman Primates Nonhuman primates are the closest relatives to humans and therefore our most evolu... 05月03日 发表评论 阅读全文
生物知识 Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells The development of small-interfering RNA (siRNA)-mediated gene-silencing strategie... 05月03日 发表评论 阅读全文
生物知识 Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval In this chapter, postembedding immunoelectron microscopy methods for studies of co... 05月03日 发表评论 阅读全文
生物知识 Experimental Approaches to the Study of Human Primordial Germ Cells The survival, proliferation, and differentiation of primordial germ cells in the m... 05月03日 发表评论 阅读全文
生物知识 ELISA实验在生物医学研究中的应用案例 案例背景随着全球癌症发病率的不断上升,早期发现、早期诊断和早期治疗成为提高癌症患者生存率的关键。肿瘤标志物作为肿瘤发生发展过程中由肿瘤细胞或宿主细胞产生并释放到体液... 05月03日 发表评论 阅读全文
生物知识 Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection Helicobacter pyloriis a Gram-negative bacteria that infects the human stomach of h... 05月03日 发表评论 阅读全文
生物知识 The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability We describe here the use of the xCELLigence system for label-free and real-time mo... 05月03日 发表评论 阅读全文