生物知识 Isolation of Total RNA from Bacteria The susceptibihty of RNA to degradation by exogenous and endogenous RNase activity... 05月03日 发表评论 阅读全文
生物知识 Real-Time RT-PCR for CTG Repeat-Containing Genes Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in... 05月03日 发表评论 阅读全文
生物知识 A Transplant Model for Human Epidermal Skin Regeneration This protocol describes a technically simple in vivo assay of long-term skin regen... 05月03日 发表评论 阅读全文
生物知识 Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt Studies of the human genome have prompted the development of several cloning syste... 05月03日 发表评论 阅读全文
生物知识 In Vitro Assays for Neural Stem Cell Differentiation Neural stem cells (NSCs) are an undifferentiated population of cells residing in t... 05月03日 发表评论 阅读全文
生物知识 Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells The major limitations in gene transduction to embryonic stem (ES) cells are (1) lo... 05月03日 发表评论 阅读全文
生物知识 Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress The upregulation of stress proteins is an important step in the cellular defense a... 05月03日 发表评论 阅读全文
生物知识 Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T Monitoring of minimal residual disease (MRD) in patients with acute or chronic mye... 05月03日 发表评论 阅读全文
生物知识 The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Tight junctions (TJs) are intercellular structures in epithelial and endothelial c... 05月03日 发表评论 阅读全文
生物知识 培养物的污染及防止 相关专题 按现代的观念,凡是混入培养环境中对细胞生存产生有害的成分和造成细胞不纯的异物都应该视为污染。根据这一概念,组织培养污染物应包括生物(真菌、细菌 、病毒和支... 05月03日 发表评论 阅读全文