一、种类与分布广泛非编码RNA种类繁多，包括tRNA、rRNA、snRNA、snoRNA、miRNA、siRNA、piRNA、lncRNA以及circRNA等多种类...
生物知识
生物知识
生物知识
Genetic-Engineered Models of Skeletal Diseases I: Collagen Type X
Formation of the vertebrate skeleton is an intricately orchestrated, multistep pro...
生物知识
Under-Agarose Chemotaxis of Dictyostelium discoideum
In the vegetative state,Dictyosteliumamoebae are chemotactic toward pterins releas...
生物知识
An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes
Within the last few years, it has become well established that a given nucleic aci...
生物知识
Routine Culture and Differentiation of Human Embryonic Stem Cells
Human embryonic stem cells provide both an in vitro model of human development and...
生物知识
High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
We have described a protocol for performing high-throughput immunofluorescence mic...
生物知识
Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
Human gene transfer with gammaretroviral, murine leukemia virus (MLV) based vector...
生物知识
Reverse Transfected Cell Microarrays in Infectious Disease Research
Several human pathogenic viruses encode large genomes with often more than 100 gen...
生物知识
Quantitating mRNAs with Relative and Competitive RT-PCR
Reverse transcription coupled with the polymerase chain reaction (RT-PCR), permits...
生物知识
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
As human embryonic stem cells (hESCs) undergo differentiation, they express genes ...
