This chapter will describe the preparation and use of a semipermeabilized (SP) cel...
生物知识
生物知识
生物知识
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein inter...
生物知识
Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Sa
Ras proteins were highly conserved during evolution. They function as a point of c...
生物知识
协助扩散
物质通过质膜进出细胞的方式之一。物质必须在载体蛋白的协助下，才能从高浓度一侧通过质膜向低浓度一侧扩散，但此种方式不消耗代谢能。协助扩散和自由扩散都是物质顺浓度梯度的...
生物知识
Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. cri
The glucosylation of 5-position of anthocyanin molecules is one of most important ...
生物知识
A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae
Critical cellular processes such as DNA replication, DNA damage repair, and transc...
生物知识
Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity
Cellular membranes, which contain abundant phospholipids, such as phosphatidylchol...
生物知识
Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
The two-hybrid system (THS) (1 ) is a molecular genetic screen that detects protei...
生物知识
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Genome sequences of many filamentous fungi are now available and additional genome...
生物知识
Control of the Interferon Response in RNAi Experiments
The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever sin...
