Knockouts by homologous recombination are frequently used to investigate the funct...
生物知识
生物知识
生物知识
限制酶和DNA 甲基化酶
限制酶特异性地结合于一段被称为限制酶识别序列的特殊DNA 序列之内或其附近的特异位点上，井在此切割双链DNA 。它可分3 类，分别是第一型（Type I）、第二型（...
生物知识
重叠延伸PCR实验
重叠延伸PCR实验重叠延伸PCR技术（gene splicing by overlap extension PCR，简称SOEPCR）由于采用具有互补末端的引物，使...
生物知识
SDS-PAGE的主要应用有哪些？
SDS-PAGE原理蛋白质是两性电解质，在一定的pH条件下解离而带电荷。当溶液的pH大于蛋白质的等电点(pI)时，蛋白质本身带负电，在电场中将向正极移动；当溶液的p...
生物知识
Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
The green fluorescent protein (GFP) from the Pacific Northwest jellyfishAequorea v...
生物知识
Measurement of Geminin Activity in Xenopus Egg Extracts
Geminin is an unstable protein that inhibits DNA replication by interfering with t...
生物知识
Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
The process of bringing a new pharmacologically active drug to market is laborious...
生物知识
On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
This chapter reviews the theory and methodological developments of on-line concent...
生物知识
Scale-Up of Suspension and Anchorage-Dependent Animal Cells
In this chapter, scale-up is described in a laboratory context (10–20 L), but the ...
生物知识
Conventional Micropipette-Based Patch Clamp Techniques
The patch clamp technique revolutionized the study of ion channels and is consider...
