生物知识 原代培养(primary culture) 原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此,较为严格地说是指成功传代之前的培养,此时的细胞保持原有细胞的基本性质,如果是正常细胞,仍然保留二倍体... 05月04日 发表评论 阅读全文
生物知识 Magnetic Resonance Imaging Analysis of Embryos Analyzing phenotypic effects in embryos produced with directed gene manipulation i... 05月04日 发表评论 阅读全文
生物知识 Isolation and Purification of Vertebrate DNAs Isolation of DNA from surrounding tissues is a crucial initial step in any molecul... 05月04日 发表评论 阅读全文
生物知识 COPⅡ被膜小泡(COPⅡ coated vesicle) 由外被蛋白Ⅱ(coat protein Ⅱ, COP Ⅱ )包裹的小泡。外被蛋白是一个大的复合体,称为外被体(coatomer)。这种类型的小泡介导非选择性运输, ... 05月04日 发表评论 阅读全文
生物知识 Profiling the Metabolic Signature of Senescence Aging is a complex process, which involves changes in different cellular functions... 05月04日 发表评论 阅读全文
生物知识 In Situ Hybridization for Metalloproteinases and Their Inhibitors The imminent completion of the human and mouse genome projects will reveal all met... 05月04日 发表评论 阅读全文
生物知识 Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells This chapter describes the analysis of long- and short-lived plasma cells on tissu... 05月04日 发表评论 阅读全文
生物知识 Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cells... 05月04日 发表评论 阅读全文
生物知识 Detection by TUNEL labeling In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannheim ... 05月04日 发表评论 阅读全文
生物知识 Identification of Mutations in the Kir6.2 Subunit of the KATP Channel The beta-cell ATP-sensitive potassium channel is a key component of stimulus —secr... 05月04日 发表评论 阅读全文