将互相匹配的DNA末端连接起来可以产生新的酶切位点。这些DNA末端可以通过以下方法获得： 1. 补平5′突出的粘性末端产生平末端 2. 双酶切产生的平末端 3. 双...

A Rapid CTAB DNA Isolation TechniqueUseful for RAPD Fingerprinting and Other PCR A...

1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtOH ...

UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/...

Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. ...

1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract a...

Prepare a 100 µl reaction mixture containing the DNA of interest in the appropriat...

A simple, reversible way to a stop restriction reaction is by adding EDTA, which c...

1) Grow 3-5 ml over-night E. coli cultures containing your plasmid2) Spin down 1.5...

在收集到生物材料之后，最好能即可进行RNA制备工作。若需暂时储存，则应以液氮将生物材料急速冷冻后，储存于-80℃冷冻柜。在制备RNA时，将储存于冷冻柜的材料取出，立...