Mammary reconstitution assays can be used to measure the stem cell frequency withi...
生物知识
生物知识
生物知识
Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
Wnts play a central role in the development of many cells and tissue types in all ...
生物知识
Analysis of Myristoylated and Palmitoylated Src Family Proteins
Several hundred viral and cellular proteins have been shown to be covalently modif...
生物知识
Generation and Establishment of Murine Adherent Cell Lines
We describe a method to derive cell lines and clones from cells of the murine midg...
生物知识
核酶(ribozyme)
核酶一词用于描述具有催化活性的RNA, 即化学本质是核糖核酸(RNA), 却具有酶的催化功能。核酶的作用底物可以是不同的分子, 有些作用底物就是同一RNA分子中的某...
生物知识
Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identifica
The nuclear envelope (NE) is a double membrane system that is both a part of the e...
生物知识
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Co
Adhesive interactions of cells with blood vessel walls under flow conditions are c...
生物知识
爬杆回避实验
原理以灯光（和/或声音）和电击为联合刺激，使实验动物由被动回避到建立主动的条件反射。记录此条件反射建立过程中的主动回避反应指标可反应实验动物的学习、记忆能力的变化。...
生物知识
旷场实验
原理旷场实验（open field test)又称敞箱实验，是评价实验动物在新异环境中自主行为、探究行为与紧张度的一种方法。以实验动物在新奇环境之中某些行为的发生频...
生物知识
旷场实验（OF）
原理旷场实验是在旷场反应箱中，评估动物运动和探索能力的行为测试之一。一般来说有两个因素决定了动物在其中的行为：一是啮齿动物本性的积极探索驱动力，以探索新的环境（食物...
